84-1085-63　Nicotinic Receptor, alpha 3 50ul　N2565-10C

特徴

• Acetylcholine receptors (AChRs) mediate synaptic transmission at the neuromuscular junction.
• The channel-linked AChR that mediates rapid, excitatory actions of acetylcholine is called nicotinic AChR (nAChR) because it can be activated by nicotine.
• The non-channel linked AChR that medicates the slow actions of acetylcholine, which can be either inhibitory or excitatory, is called muscarinic AChR (mAChR) because it can be activated by muscarine.
• The nAChRs fall into two subclasses, the muscle-type and the neuronal-type.
• The muscle-type receptor is a pentameric glycoprotein of five membrane spanning subunits, (2 alpha 1’s, beta 1, gamma or epsilon and delta) which form a ligand gated ion channel.
• The neuronal nAChR is also a pentamer, but unlike the muscle-type, is made up of only two general types of subunits, alpha and beta of which multiple subtypes have been described (alpha 2 to alpha 8 and beta 2 to beta 4).
• Applications:Suitable for use in ELISA, Western Blot and Immunocytochemistry.
• Other applications not tested.
• Recommended Dilutions:Immunocytochemistry: 20ug/ml.
• Cells and tissues should be fixed using PLP fixative and ABC detection
• Western Blot: 25ug/ml.
• Recognizes single band at 59kD
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.