Applications:Suitable for use in ELISA, Western Blot, Immunocytochemistry and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:400 using whole rat brain homogenate and cultured cells known to express the M5 receptorImmunohistochemistry: 1:400 using PLP-fixed rat brain sectionsOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Grade：Serum
Purity：Serum
Form：Supplied as a lyophilized powder. Reconstitute with 0.1ml PBS containing 10mg/ml BSA and 40-50% glycerol.
Specificity：Recognizes M5 Muscarinic Receptor. Reactivity: M5 Muscarinic Receptor (aa519-531): 100%; M5 Muscarinic Receptor: 75%. Does not react with M1 Muscarinic Receptor (451-460), M1 Muscarinic Receptor, M2 Muscarinic Receptor (457-466), M2 Muscarinic Receptor, M3 Muscarinic Receptor (580-589), M3 Muscarinic Receptor, M4 Muscarinic Receptor (469-478) and M4 Muscarinic Receptor. Specific for the COOH terminal of the M5 receptor.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Immunogen：Peptide analog of the carboxyl terminal of the muscarinic M5 receptor covalently attached onto a carrier protein.
