The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors.
The functional diversity of these receptors is defined by the binding of acetylcholine and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation.
Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system.
Applications:Suitable for use in ELISA, Western Blot and Immunocytochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:400 using whole rat brain homogenate Immunocytochemistry: 1:500 stains fixed rat brain sections using ABC tequniquesOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile PBS, 10mg/ml BSA.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Grade：Serum
Purity：Serum
Form：Supplied as a lyophilized powder. Reconstitute with 100ul sterile PBS, 10mg/ml BSA.
Specificity：Recognizes M4 Muscarinic Receptor (469-478). Reactivity of M4 Muscarinic Receptor is 70%. Does not react with M1 Muscarinic Receptor (451-460), M1 Muscarinic Receptor, M2 Muscarinic Receptor (457-466), M2 Muscarinic Receptor, M3 Muscarinic Receptor (580-589), M3 Muscarinic Receptor, M5 Muscarinic Receptor (519-531) and M5 Muscarinic Receptor. Specific for the COOH terminal of the M4 receptor.
Isotype：IgG
Calc Applications Abbrev：E IC WB
Immunogen：Peptide analog of the carboxyl terminal of the muscarinic M4 receptor covalently attached onto a carrier protein.
