Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: Further dilute stock solution 1:8 with additional buffer (see WB protocol).
This antibody should be sufficient for at least 20 lanes
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile PBS containing 10mg/ml BSA.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：5ml
Host：rabbit
Grade：Highly Purified
Purity：Purified by precipitation, dialysis and chromatography.
Form：Supplied as a lyophilized powder. Reconstitute with 5.0 ml of sterile PBS containing10 mgm/ml BSA.
Specificity：Recognizes M3 Muscarinic Receptor (580-589) (100%) and full-length M3 Muscarinic Receptor (70%). Does not react with M1 Muscarinic Receptor (451-460), M1 Muscarinic Receptor, M2 Muscarinic Receptor (457-466), M2 Muscarinic Receptor, M4 Muscarinic Receptor (469-478), M4 Muscarinic Receptor, M5 Muscarinic Receptor (519-531) and M5 Muscarinic Receptor. Has been found to stain specifically the M3 receptor in Western immunoblots of whole rat brain homogenates.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Immunogen：Peptide analog of the carboxyl terminal of the M3 receptor covalently attached onto a carrier protein.
