The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors.
The functional diversity of these receptors is defined by the binding of acetylcholine and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation.
Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system.
The muscarinic cholinergic receptor 3 controls smooth muscle contraction and stimulates secretion in glandular tissue.
Applications:Suitable for use in ELISA, Western Blot and Immunocytochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:500Immunocytochemistry: 1:2500Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS, 10mg/ml BSA.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months after receipt at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Grade：Serum
Purity：Serum
Form：Supplied as a lyophilized powder. Reconstitute with 100ul sterile PBS containing 10mg/ml BSA.
Specificity：Recognizes the C-terminal of the M3 receptor. Recognizes M3 Muscarinic Receptor (580-589) (100%) and full-length M3 Muscarinic Receptor (70%). Does not react with M1 Muscarinic Receptor, M2 Muscarinic Receptor, M4 Muscarinic Receptor or the M5 Muscarinic Receptor. Species Crossreactivity: human and rat
Isotype：IgG
Calc Applications Abbrev：E IC WB
Calc Crossreactivity：Hu Rt
Immunogen：Synthetic peptide containing the C-terminal of the muscarinic M3 receptor (aa580-589) covalently attached onto a carrier protein.
