Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000 using whole rat brain homogenate
Immunohistochemistry: 1:5000.
Stains PLP-fixed rat brain sections
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
Reconstitute with PBS containing 10mg/ml BSA, aliquot, and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Grade：Serum
Purity：Serum
Form：Supplied as a lyophilized powder. Reconstitute with 0.1ml of 10mg/ml BSA in PBS.
Specificity：Recognizes rat M2 Muscarinic Receptor (457-466) and the reactivity of M2 Muscarinic Receptor is 50%. Does not react to M1 Muscarinic Receptor (451-460), M1 Muscarinic Receptor, M3 Muscarinic Receptor (580-589), M3 Muscarinic Receptor, M4 Muscarinic Receptor (469-478), M4 Muscarinic Receptor, M5 Muscarinic Receptor (519-531) and M5 Muscarinic Receptor. This antiserum is specific for the COOH terminal of the M2 receptor.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Rt
Immunogen：Peptide analog of the carboxyl terminal of the muscarinic M2 receptor covalently attached onto a carrier protein.
