84-1085-50　Muscarinic Receptor M1 (Cholinergic Muscarinic Receptor M1, Chrm1) 100ul　M9520-03A

特徴

• The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors.
• The functional diversity of these receptors is defined by the binding of acetylcholine and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration and potassium channel mediation.
• Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system.
• The muscarinic cholinergic receptor 1 is involved in mediation of vagally-induced bronchoconstriction and in the acid secretion of the gastrointestinal tract.
• The gene encoding this receptor is localized to 11q13.
• Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 1:800 using whole rat brain homogenate
• Immunohistochemistry: 1:5000 using ABC techniques on PLP fixed rat brain sections Optimal dilutions to be determined by the researcher.
• Recommended Secondary Antibodies:I1904-39: IgG, X-Adsorbed (HRP) Pab Gt x RbI1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt x RbI1904-46Q: IgG, H&L (HRP) Pab Gt x Rb
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with sterile PBS, 10mg/ml BSA.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.