Applications:Suitable for use in ELISA, Western Blot and Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000Immunocytochemistry: 1:500Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile BSA in PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size:100ul
Host:rabbit
Source Antibody:human
Grade:Serum
Purity:Serum
Form:Supplied as a lyophilized powder. Reconstitution with 0.1ml of 10mg/ml BSA in PBS.
Specificity:Recognizes human Leptin and Leptin (aa1-24). Species Crossreactivity: rat ~90%. Does not react to human Epidermal Growth Factor, Insulin, Insulin-like Growth Factor 1, Transforming Growth Factor-alpha and rat nsulin-like Growth Factor 2. Specific for the amino terminal of human Leptin, crossreacts with the amino terminal of rat Leptin.
Isotype:IgG
Calc Applications Abbrev:E IC WB
Calc Crossreactivity:Hu
Immunogen:Synthetic peptide corresponding to aa1-24 of human Leptin (carrier protein).