Applications:Suitable for use in RIA, ELISA, Western Blot and Immunoprecipitation.
Other applications not tested.
Recommended Dilution:Immunoprecipitation: 1:500- 1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a lyophilized powder. Reconstitution with 0.1ml of 10mg/ml BSA in PBS.
Specificity：Recognizes Leptin (human) and Leptin (human) [131-145]. The reactivity of Leptin (rat) and Leptin (mouse) is ~75%. Does not react to Epidermal Growth Factor (human), Insulin-like Growth Factor 1 (human), Insulin-like Growth Factor 2 (rat), Parathyroid hormone (human) and Transforming growth factor-alpha (human). Specific for the COOH terminal of the human Leptin.
Isotype：IgG
Calc Applications Abbrev：E IP RIA WB
Calc Crossreactivity：Hu
Immunogen：Peptide analog of the carboxyl terminal of the human Leptin covalently attached onto a carrier protein.
