Human Leptin (Anti-Obesity Protein) is the human homolog of murine Leptin, the protein product of the ob (obese) gene in mice which seems to be involved in appetite control.
The murine ob gene is expressed in adipose tissue and is thought to regulate the body's fat stores.
It targets the central nervous system, particularly hypothalamus, affecting food intake.
Mice with the ob/ob genotype also develop a form of diabetes similar to type II (non-insulin dependent) diabetes.
Applications:Suitable for use in RIA, ELISA, Western Blot, Immunoprecipitation and Cell Staining.
Other applications not tested.
Recommended Dilutions:Immunoprecipitation: 1:500-1:5000; Quantitatively immunoprecipitates Leptin depending upon the amount of Leptin in a sample.
Tested using double antibody precipitation and magnetic bead techniques
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS, 10mg/ml BSA.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a lyophilized powder. Reconstitue with 100ul sterile PBS, 10mg/ml BSA.
Specificity：Recognizes human Leptin (aa1-24, amino terminal). Species Crossreactivity: mouse, monkey, porcine and rat, ~75%. Does not react to human epidermal growth factor, human insulin-like growth factor 1, rat insulin-like growth factor 2, human parathyroid hormone or human transforming growth factor-alpha.
Isotype：IgG
Calc Applications Abbrev：E IP RIA WB
Calc Crossreactivity：Hu Mk Mo Po Rt
Immunogen：Synthetic peptide corresponding to aa1-24, of human Leptin covalently attached onto a carrier protein.
