Applications:Suitable for use in RIA, ELISA, Western Blot and Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500Immunocytochemistry: 1:800Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a lyophilized powder. Reconstitution with 0.1ml of 10mg/ml BSA in PBS.
Specificity：Recognizes Leptin (Human). The reactivity of Leptin (Rat) and Leptin (Mouse) is ~75%. Does not react to Epidermal growth factor (Human), Insulin (Human), Insulin-like Growth Factor 1 (Human), Insulin-like Growth Factor 2 (Rat) and Transforming growth factor-alpha (Human). Specific for the COOH terminal.
Isotype：IgG
Calc Applications Abbrev：E IC RIA WB
Calc Crossreactivity：Hu
Immunogen：Peptide analog of the carboxyl terminal of human leptin covalently attached onto a carrier protein.
