Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:8Optimal dilutions to be determined by the researcher.
Control Peptide:I0800-08Q1: Inducible Nitric Oxide Synthase, Control Peptide (iNOS, iNOS 2, mNOS); 150nM.
Reconstitute with 600ul stock antibody solution for blocked antibody control.
Recommended Positive Control:A-172 cells
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile 40-50% glycerol, TBS/Tween®-20, 1% normal goat serum.
Aliquot and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：5ml
Host：rabbit
Source Antibody：human
Grade：Highly Purified
Purity：Purified by precipitation, dialysis and chromatography.
Form：Supplied as a lyophilized powder. Reconstitution: Reconstitute with 5ml of sterile 40-50% glycerol, TBS/Tween®-20, 1% normal goat serum.
Specificity：Recognizes hiNOS (1137-1153). The reactivity of rhiNOS (Type 2) is ~50%. Does not react to rhnNOS (Type 1) or rheNOS (Type 3). Has been found to stain specifically hiNOS in Western immunoblots of A-172 cells induced to produce hiNOS.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Peptide analog of the carboxyl terminal of hiNOS covalently attached onto a carrier protein.
