Applications:Suitable for use in ELISA, Western Blot and Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1600Immunocytochemistry: 1:800Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size:50ul
Host:rabbit
Source Antibody:human
Grade:Purified
Purity:Purified immunoglobulin
Form:Supplied as a lyophilized powder. Reconstitution with 0.1ml of 10mg/ml BSA in PBS.
Specificity:Recognizes Epidermal Growth Factor (Human). The reactivity of EGF (rat) is 90% and EGF (mouse) is 1%. Does not react to IGF-1 (human), IGF-2 (rat), TGF alpha (human), ACTH (human, 1-39), Calcitonin (human), PTH (bovine, 1-84), Somatostatin and Vasoactive Intestinal Peptide. Specific for hEGF and is suitable for immunocytochemical and Western immunoblot detection of this growth factor.
Isotype:IgG
Calc Applications Abbrev:E IC WB
Calc Crossreactivity:Hu
Immunogen:Synthetic human epidermal growth factor (hEGF) covalently attached onto a carrier protein.