84-1085-12　Dopamine Receptor, D2L 5ml　D9000-09E

特徴

• Dopamine is an endogenous catecholamine that influences many cellular activities, including behavior, hormone synthesis and release, blood pressure and intracellular ion transport.
• A family of at least 5 Dopamine Receptors (DR) genes, D1-D5, have been identified based upon the amino acid identity, pharmacological specificity and effector responses.
• DR have been classified into either the D1-like (D1, D1B, and D5) or D2-like (D2, D3, and D4).
• The two isoforms of D2R, D2 long (D2L) and short D2S), are encoded by splice variants of a single gene and differ only by the presence of an additional 29aa in the intracellular domain 3 of the D2 long form.
• It may play a role in the coupling of the receptor to G-proteins.
• All members of this family have similar structure and contain 7 putative transmembrane domains.
• A given cell or tissue may express more than one DR.
• Specific radioligands do not exist that can differentiate between these DR.
• Therefore, specific antibodies are needed to distinguish, localize, and document changes in DR levels in cells and tissues under various normal and pathological conditions.
• Applications:Suitable for use in ELISA, Western Blot and Immunocytochemistry.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 1:8Optimal dilutions to be determined by the researcher.
• Control Peptide (Supplied):D9000-09E1: Dopamine Receptor, D2L, Control Peptide
• Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
• Reconstitute with sterile PBS, 10mg/ml BSA.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.