D(2) dopamine receptor is one of five (D1 to D5) receptors for dopamine.
Its activity is mediated by G proteins which inhibit adenylyl cyclase.
It interacts with GPRASP1, neurabin-2, CLIC6, CADPS and CADPS2.
It is a transmembrane protein that belongs to the G-protein coupled receptor 1 family.
The unprocessed precursor has a length of 443 amino acids and an estimated molecular mass of 50.6kDa.
Genetic variations in DRD2 may determine the genetic susceptibility to alcoholism.
A missense mutation in this gene causes myoclonus dystonia and several other defects in this gene are thought to be involved in psychiatric disorders, especially in schizophrenia.
Applications:Suitable for use in ELISA, Western Blot and Immunocytochemistry.
Other applications not tested.
Recommended Dilutions:Immunocytochemistry: 1:5000 on PLP fixed rat brain sectionsWestern Blot: 1:800Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile PBS, 10mg/ml BSA.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Grade：Serum
Purity：Serum
Form：Supplied as a lyophilized powder. Reconstitute with l00ul sterile PBS,10mg/ml BSA.
Specificity：Recognizes D2 Dopamine Receptor (272-282). The reactivity of D2S Dopamine Receptor is ~70% and the reactivity of D2L Dopamine Receptor is ~60%. No crossreactivity wirth D1 Dopamine Receptor (9-21), D1 Dopamine Receptor, D3 Dopamine Receptor (2-10), D3 Dopamine Receptor, D4 Dopamine Receptor (176-185), D4 Dopamine Receptor, D5 Dopamine Receptor (23-35) and D5 Dopamine Receptor. Highly specific for this peptide sequence and is suitable for immunocytochemical detection of both the short and the long forms of the D2 dopamine receptor.
Isotype：IgG
Calc Applications Abbrev：E IC WB
Calc Crossreactivity：Rt
Immunogen：D2 (272-282) covalently attached onto a carrier protein.
