84-1084-88　［取扱停止］EBP50 (ERM (Ezrin-Radixin-Moesin) Binding Phosphoprotein of 50kD, Na(+)/H(+) Exchange Regulatory Cofactor NHE-RF1, NHERF, NHERF1, NHERF-1, NPHLOP2, Regulatory Cofactor of Na(+)/H(+) Exchanger, Sodium Hydrogen Exchanger Regulatory Factor 1, Solute Carrier Family 9 Isoform A3 Regulatory Factor 1, SLC9A3R1) (Biotin) 100ul　E1050B

特徴

• EBP 50 [ERM (ezrin-radixin-moesin) binding phosphoprotein of 50kD] is a PDZ containing protein that is involved in the linkage of integral membrane proteins to the cytoskeleton.
• EBP50 contains two tandem PDZ domains followed by a carboxy-terminal sequence that binds to members of the ERM family of membrane-cytoskeleton adaptors.
• The PDZ domains within EBP50 bind to the carboxy termini of such target proteins as beta2 adrenergic receptor, platelet-derived growth factor receptor (PDGFR), and the cystic fibrosis conductance regulator (CFTR).
• The PDZ domains are also believed to be involved in the oligomerization of EBP 50 to form multiprotein complexes which helps to facilitate the formation of functional signalling complexes.
• EBP 50 has been shown to link integral membrane proteins to the cytoskeleton by binding to members of the ERM family of proteins, which bind to F-actin.
• Through these interactions, EBP 50 is implicated in the localization of interactive groups of proteins into subcellular domains and in the regulation of activity of those interacting proteins.
• Immunohistochemical studies have shown that EBP 50 is found exclusively at the apical membranes of epithelial cells.
• Application(s):Suitable for use in Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1ug/ml Optimal dilutions to be determined by the researcher.
• Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
• For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
• Aliquots are stable at -20°C for 12 months after receipt.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Note: Applications are based on unconjugated antibody.