Survivin, otherwise known as TIAP/BIRC5, is a novel inhibitor of apoptosis (IAP) protein, and component of the chromosomal passenger complex, which plays a key role in the regulation of mitosis.
Survivin is only expressed during the G2-M cell cycle phase, and is predominantly expressed in fetal tissues, and in many human cancers, including adenocarcinomas and high-grade lymphomas.
The lack of survivin expression by normal non-malignant cells, makes survivin an important therapeutic target for cancer.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:Peptide ELISA: 1:16,000Western Blot: 0.01-0.5ug/ml, detects a band of ~18kD in MOLT4 cell lysates
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.
Specificity：Recognizes human Survivin. This antibody is expected to recognize the reported isoforms 1 and 3 (NP_001159.2 and NP_001012271.1 resp.)
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to CVRRAIEQLAAMD from the C-terminal region of human Survivin
