84-1084-70　［取扱停止］SLP 76 (LCP2, SLP76, SLP-76, lymphocyte cytosolic protein 2 (SH2 domain containing leukocyte protein of 76kD), 76kD tyrosine phosphoprotein, SH2 domain-containing leukocyte protein of 76kD, lymphocyte cytosolic protein 2 (SH2 domain containing leukocyte protein of 76kD)) 100ug　S1014-20K

特徴

• Alternate Name: LCP2, SLP76, SLP-76, lymphocyte cytosolic protein 2 (SH2 domain containing leukocyte protein of 76kD), 76 kD tyrosine phosphoprotein, SH2 domain-containing leukocyte protein of 76kD, lymphocyte cytosolic protein 2 (SH2 domain containing leukocyte protein of 76kD)
• Domain Information: KOG1984: Vesicle coat complex COPII, subunit SFB3 [Intracellular trafficking, secretion, and vesicular transport]; smart00252: Src homology 2 domains; smart00454: Sterile alpha motif
• Gene Ontology Terms: immune response; intracellular signaling cascade; protein binding; transmembrane receptor protein tyrosine kinase signaling pathway
• Applications:Suitable for use in ELISA and Western Blot.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:32,000Western Blot: 0.5-2ug/ml ~75kD band observed in Jurkat lysates
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.
• Peptide Blocking:Corresponding peptide is available for Peptide Blocking studies.
• See S1014-20K-P.
• Antibody is typically 0.5mg/ml and peptide is supplied as a 100ul pellet.
• When peptide is reconstituted in 200ul water, the concentration would also be 0.5mg/ml.
• To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed).
• Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature.
• It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min.
• Then incubate and develop the two blots in parallel.