Applications:Suitable for use in Immunohistochemistry, ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:Peptide ELISA: 1:64,000Western Blot: 0.1-0.3ug/ml, ~48kD band observed in Human Liver and HeLa lysates, while approx.
45kD observed in lyates of cell lines A549 and HepG2 (calculated MW of 45.1kD according to NP_000593.1)
Immunohistochemistry: 4-6ug/ml.
In paraffin embedded Human Placenta shows strong staining of cytotrophoblastsOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.
Specificity：Recognizes human PAI1/SERPINE1.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu
Immunogen：Peptide with sequence C-GFKIDDKGMAPALRH, corresponding to the internal region of human PAI-1 (NP_000593.1). Species sequence homology: canine, chimpanzee
