Applications:Suitable for use in Peptide ELISA, Western Blot, and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Peptide ELISA:1:64000Western Blot: 0.01-0.03ug/ml ~35kD band observed in Human Tcell line MOLT4 lysates (MW of 33.2kD)
Immunohistochemistry: 2-5ug/ml In paraffin embedded tonsil, stains clusters of cells some of which are likely to be plasma cells.
In the interfollicular area occasional strongly stained areas.
Epithelium is weakly positive.
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
Peptide Blocking:Corresponding peptide is available for Peptide Blocking studies.
See M9755-09A-P.
Antibody is typically 0.5mg/ml and peptide is supplied as a 100ul pellet.
When peptide is reconstituted in 200ul water, the concentration would also be 0.5mg/ml.
To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed).
Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature.
It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min.
Then incubate and develop the two blots in parallel.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.
Specificity：Recognizes human MYD88. Species sequence homology: rat, mouse, canine, and chimpanzee.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Ca Hu Mo Rt
Immunogen：Synthetic peptide, C-IKYKAMKKEFP, internal region of human MyD88.
