Alternate Name: KLRK1, killer cell lectin-like receptor subfamily K, member 1, HGNC:18788, D12S2489E, KLR, NKG2-D, NKG2D, NK cell receptor D, NKG2-D type II integral membrane protein
Western Blot: Approx 35kD band observed in Human Lymph Node and Human Spleen lysates (calculated MW of 25.3kD according to NP_031386.1).
The observed molecular weight is explained by glycosylation (Han et al, Blood.
2004 Nov 1;104(9):2858-66.
Epub 2004 Jul 6; PMID: 15238421).
Recommended concentration: 0.1-0.3ug/ml.
Staining (0.3ug/ml) of Human Spleen lysate (RIPA buffer, 35ug total protein per lane).
Primary incubated for 1 hour.
Detected by western blot using chemiluminescence
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
Peptide Blocking:Corresponding peptide is available for Peptide Blocking studies.
See K1893-28-P.
Antibody is typically 0.5mg/ml and peptide is supplied as a 100ul pellet.
When peptide is reconstituted in 200ul water, the concentration would also be 0.5mg/ml.
To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed).
Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature.
It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min.
Then incubate and develop the two blots in parallel.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.
Specificity：Recognizes Human, Mouse, Rat KLRK1/NKG2D
