Domain Information: COG5048, FOG: Zn-finger [General function prediction only]
Gene Ontology Terms: DNA binding; regulation of transcription, DNA-dependent; nucleus
Application(s): Suitable for use in ELISA, Western Blot.
Other applications not tested
Western blot: Approx 50kD band observed in Human Kidney lysates (calculated MW of 39.3 acording to NP_009181.2) .
In glioblastomas this protein was reported to run at appr.
60kD (Ding et al .
J Biol Chem.
2005 Feb 25;280(8):6802-15; PMID: 15557280).
Recommended for use at 1-3ug/ml.
Staining (2ug/ml) of Human Kidney lysate (RIPA buffer, 30ug total protein per lane).
Primary incubated for 1 hour.
Detected by western blot using chemiluminescence
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
Peptide Blocking:Corresponding peptide is available for Peptide Blocking studies.
See K1891-50A-P.
Antibody is typically 0.5mg/ml and peptide is supplied as a 100ul pellet.
When peptide is reconstituted in 200ul water, the concentration would also be 0.5mg/ml.
To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed).
Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature.
It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min.
Then incubate and develop the two blots in parallel.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.
