84-1084-31　HADH2 (HADH2, ABAD, ERAB, MHBD, HSD17B10, 17b-HSD10, hydroxyacyl-Coenzyme A dehydrogenase, type II, type 10 17b-HSD, AB-binding alcohol dehydrogenase, type 10 17beta-hydroxysteroid dehydrogenase) 100ug　H1500-02

特徴

• Alternate Name: HADH2, ABAD, ERAB, MHBD, HSD17B10, 17b-HSD10, hydroxyacyl-Coenzyme A dehydrogenase, type II, type 10 17b-HSD, AB-binding alcohol dehydrogenase, type 10 17beta-hydroxysteroid dehydrogenase
• Gene Ontology Terms: 3-hydroxyacyl-CoA dehydrogenase activity; 7-alpha-hydroxysteroid dehydrogenase activity; lipid metabolism; metabolism; mitochondrion; oxidoreductase activity; plasma membrane
• Application(s):Peptide ELISA: antibody detection limit dilution 1:32,000.
• Western Blot: 0.3-2ug/ml.
• Approx 25kD band observed in Human Brain lysates (calculated MW of 26.9kD according to NP_004484).
• A minor band of unknown identity was also consistently observed at appr 38kD.
• This band was successfully blocked by incubation with the immunizing peptide.
• Staining (0.3ug/ml) of Human Brain lysate (RIPA buffer, 30ug total protein per lane).
• Primary incubated for 1 hour.
• Detected by western blot using chemiluminescence
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.
• Peptide Blocking:Corresponding peptide is available for Peptide Blocking studies.
• See H1500-02-P.
• Antibody is typically 0.5mg/ml and peptide is supplied as a 100ul pellet.
• When peptide is reconstituted in 200ul water, the concentration would also be 0.5mg/ml.
• To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed).
• Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature.
• It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min.
• Then incubate and develop the two blots in parallel.