The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD).
The enzyme exists as a tetramer of identical chains.
Many pseudogenes similar to this locus are present in the human genome.
GAPDH is constitutively expressed in almost all tissues at high levels.
It is therefore a useful marker when a loading/positive control is required in Western Blot.
Applications:Suitable for use in ELISA and Western Blot.
~35kDa band observed in lysates of Human Tonsil, Mouse Spleen, and cell lines HeLa, A431 and 293 (calculated MW of 36.1kDa according to NP_002037)Immunohistochemistry: 0.3ug/ml, In paraffin embedded Human Tonsil, staining of cells in germinal centre, mantle zone, interfollicular area, epithelium and endothelium is observed.
Recommended concentration
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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Peptide Blocking:Corresponding peptide is available for Peptide Blocking studies.
See G8140-01D-P.
Antibody is typically 0.5mg/ml and peptide is supplied as a 100ul pellet.
When peptide is reconstituted in 200ul water, the concentration would also be 0.5mg/ml.
To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed).
Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature.
It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min.
Then incubate and develop the two blots in parallel.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Specificity：Recognizes human GAPDH at ~35kD. Species Crossreactivity: mouse
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to C-HQVVSSDFNSDT, from the C Terminus of human GAPDH (NP_002037.2). Species Sequence Homology: canine
