FOXP3 (a 431 amino acid protein) is a member of the forkhead/winged-helix family of transcriptional regulators and is highly conserved across mammals.
FOXP3 is essential for normal immune homeostasis.
FOXP3 is stably and constitutively expressed at a high level in CD25 + CD4 positive regulatory T cells, at low level in CD4 positive/CD25 negative cells, and is absent in CD4 negative/CD8 positive T cells.
FOXP3 may be a master regulatory gene and a more specific marker of regulatory T cells than other T cells.
Applications:Suitable for use in ELISA, Western Blot, Flow Cytometry, Immunofluorescence/Immunocytochemistry and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:8000Western Blot: 1-3ug/ml ~47kD band observed in human muscle lysates and in MOLT4 cell line lysates Immunohistochemistry (Parrafin): 0.3-1ug/ml Requires HIER using Tris-EDTA buffer pH 9.0 (Human tonsil, stains nuclei of some cells in the interfollicular area)Flow Cytometry: 10ug/ml Jurkat cellsImmunofluorescence (IC): 10ug/ml U2OS cellsImmunofluorescence (IC): 2-4ug/ml overnight human blood cellsOptimal dilution to be determined by researcher.
Negative Conrol (WB): Human pancreas lysates
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by ammonium sulfate precipitation followed by affinity chromatography
Form：Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Specificity：Recognizes human FOXP3.
Isotype：IgG
Calc Applications Abbrev：E FC IC IF IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to SQRPSRCSNPTPGP from the C-Terminus of human FOXP3.
