Catechol-O-methyltransferase catalyzes the transfer of a methyl group from S-adenosylmethionine to catecholamines, including the neurotransmitters dopamine, epinephrine, and norepinephrine.
This O-methylation results in one of the major degradative pathways of the catecholamine transmitters.
In addition to its role in the metabolism of endogenous substances, COMT is important in the metabolism of catechol drugs used in the treatment of hypertension, asthma, and Parkinson disease.
COMT is found in two forms in tissues, a soluble form (S-COMT) and a membrane-bound form (MB-COMT).
The differences between S-COMT and MB-COMT reside within the N-termini.
Several transcript variants are formed through the use of alternative translation initiation sites and promoters.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilutions: Peptide ELISA: 1:32,000Western Blot: 0.5-2ug/ml, ~60kD and 70kD bands observed in lysates of cell line HeLa (calculated MW of 64.5kD according to NP_542158.1 and 68.7kD according to NP_000953.2)Immunohistochemistry: 3-5ug/ml, in paraffin embedded Human Heart shows patchy staining of myocardial fibers in longitudinal sectionOptimal dilutions to be determined by the researcher.
Peptide Blocking:Corresponding peptide is available for Peptide Blocking studies.
See C7904-73C-P.
Antibody is typically 0.5mg/ml and peptide is supplied as a 100ul pellet.
When peptide is reconstituted in 200ul water, the concentration would also be 0.5mg/ml.
To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed).
Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature.
It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min.
Then incubate and develop the two blots in parallel.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Specificity：Recognizes human COX1. This antibody is expected to recognize both human isoforms of this protein according to NP_000953.2 and NP_542158.1.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to C- QDDGPAVERPSTEL from the C-terminus of human COX1. Species sequence homology: mouse.
