84-1084-03 Bif-1 (Bax interacting factor-1, SH3GLB1; Bif-1; CGI-61; KIAA0491; SH3-domain GRB2-like endophilin B1; endophilin B1, SH3-containing protein SH3GLB1; SH3-domain, GRB2-like, endophilin B1; SH3-containing protein SH3GLB1 CGI-61 protein endophilin B1) 100ug B1098-01B

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特徴

  • The Bcl-2 family consists of anti-apoptotic (or cell survival) genes, such as Bcl-2, Bcl-XL and pro-apoptotic (or cell death) genes including Bax and BAD.
  • In addition to its anti-apoptotic effect, Bcl-2 also inhibits progression through the cell cycle.
  • Functional interactions between family members as well as binding to other cellular proteins modulate their activities.
  • Bcl-2 family members are important in normal tissue development, homeostasis, and disease states.
  • Bcl-2 prevents cell death by forming heterodimers with Bax and Bad (1).
  • Bax promotes cell death by forming homodimers, which can be blocked by forming heterodimers with Bcl-2 or BCL-XL.
  • Recently, using yeast two-hybrid system Cuddeback et al (2) have identified a cDNA encoding a 365 amino acid protein, which interacts physically with Bax protein and influences cell life and death.
  • Bif-1 contains a Src homology 3 (SH3) domain in the C-terminus.
  • It does not contain any of the conserved Bcl-2 homology (BH) domains of the Bcl-2 family proteins.
  • Human Bif-1 protein shares 96% and 42% identity at the amino acids level with mouse and C.
  • elegans, respectively.
  • Overexpression of Bif-1 in transfected cell lines promotes Bax conformational change, caspase activation, and apoptotic cell death following growth factor withdrawal.
  • Bif-1 RNA is abundantly expressed in heart, skeletal muscle, kidney, and placenta.
  •  
  • Domain Information: BAR domain; Src homology 3 domains
  • Gene Ontology Terms:
  • Application(s):Peptide ELISA: antibody detection limit dilution 1:32,000.
  • Western Blot: Approx 38kD band observed in lysates of Human T cell line Jurkat and in Human Lung and Placenta (calculated MW of 40.8kD according to NP_057093.1).
  • Recommended concentration: 0.3-1.0 ug/ml.
  • A minor band of unknown identity was also consistently observed at 50kD in Jurkat and Placenta.
  • This band was successfully blocked by incubation with the immunizing peptide.
  • We would appreciate any feedback from people in the field-have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
  • Staining (0.3ug/ml) of Jurkat lysate (RIPA buffer, 35ug total protein per lane).
  • Primary incubated for 1 hour.
  • Detected by western blot using chemiluminescence
  • Optimal dilutions to be determined by the researcher.
  • Storage and Stability:May be stored at 4°C for short-term only.
  • For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C.
  • Aliquots are stable for at least 12 months at -20°C.
  • For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
  • Further dilutions can be made in assay buffer.
  • Peptide Blocking:Corresponding peptide is available for Peptide Blocking studies.
  • See B1098-01B-P.
  • Antibody is typically 0.5mg/ml and peptide is supplied as a 100ul pellet.
  • When peptide is reconstituted in 200ul water, the concentration would also be 0.5mg/ml.
  • To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed).
  • Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature.
  • It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min.
  • Then incubate and develop the two blots in parallel.

仕様

  • Size:100ug
  • Host:goat
  • Source Antibody:human
  • Grade:Affinity Purified
  • Purity:Purified by immunoaffinity chromatography.
  • Form:Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.
  • Specificity:Recognizes Human, Mouse, Rat, canine Bif-1 (Bax interacting factor-1, SH3GLB1)
  • Isotype:IgG
  • Calc Applications Abbrev:E WB
  • Calc Crossreactivity:Ca Hu Mo Rt
  • Immunogen:C-QKGKVPITYLELLN, C Terminus
  • Shelf Life:1year
  • Swiss Prot Number:Q9Y371
  • EU Commodity Code:30021010
  • この商品は法規制を確認しておりません。(法規制によって販売できない場合もございます)
  • 製品の仕様は予告なく変更になる場合がございます。最新仕様はメーカーホームページをご確認ください。
  •  

アズワン品番 84-1084-03
型番 B1098-01B
入り数 1個
標準価格 153,000円(税抜)
WEB価格
出荷予定日
アズワン在庫 [?]
サプライヤ在庫 [?]
数量

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