ARH is a cytosolic protein which contains a phosphotyrosine binding (PTD) domain.
The PTD domain has been found to interact with the cytoplasmic tail of the LDL receptor.
Mutations in this gene lead to LDL receptor malfunction and cause the disorder autosomal recessive hypercholesterolaemia.
Applications:Suitable for use in Peptide ELISA and Western Blot.
Other applications not tested.
Recommended Dilutions:Peptide ELISA: 1:16,000 (Detection limit dilution)Western Blot: 1-3ug/ml; 40-45kD band is observed in human liver lysate (RIPA buffer, 30ug total protein per lane).
Incubate for 1 hour.
Detected by chemiluminescence
Optimal dilutions to be determined by the researcher.
Control Peptide:A3570-65C-P: ARH Control Peptide
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Specificity：Recognizes human ARH/LDL receptor adaptor protein.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to aa9-21, DALKSAGRALIRS-C, of human ARH (NP_056442.2). Species Sequence Homology: bovine, canine, mouse, rat.
