84-1083-94　APE (APEX, APEX1, APEX nuclease multifunctional DNA repair enzyme, APX, APE1, APEN, HAP1, REF1, REF-1, AP lyase, AP endonuclease class I, DNA apurinic/apyrimidinic exonuclease/lyase) 100ug　A2298-74N

特徴

• Alternate Name: APEX, APEX1, APEX nuclease (multifunctional DNA repair enzyme), APE, APX, APE1, APEN, HAP1, REF1, REF-1, AP lyase, AP endonuclease class I, apurinic/apyrimidinic exonuclease, multifunctional DNA repair enzyme, DNA-(apurinic or apyrimidinic site) lyase, apurinic/apyrimidinic (abasic) endonuclease, deoxyribonuclease (apurinic or apyrimidinic)
• Domain Information: Exonuclease III [DNA replication, recombination, and repair]
• Gene Ontology Terms: DNA-(apurinic or apyrimidinic site) lyase activity; uracil DNA N-glycosylase activity; endodeoxyribonuclease activity; transcription co-repressor activity; 3'-5' exonuclease activity; endonuclease activity; DNA binding; transcription co-activator activity; base-excision repair; DNA repair; transcription from Pol II promoter; obsolete; nucleus
• Applications:Suitable for use in ELISA, Western Blotting.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:32000Western blot: 0.2-1ug/ml.
• ~35kD band seen in Human epidermoid fibroblast A431 and Human cervix epitheloid fibroblast HeLa lysates (calculated MW of 35.6kDa according to NP_001632.2; NP_542379.1; NP_542380.1..
• Staining (0.5ug/ml) of A431 lysate (RIPA buffer, 30ug total protein per lane).
• Primary incubated for 1 hour.
• Detected by western blot using chemiluminescence
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.
• Peptide Blocking:Corresponding peptide is available for Peptide Blocking studies.
• See A2298-74N-P.
• Antibody is typically 0.5mg/ml and peptide is supplied as a 100ul pellet.
• When peptide is reconstituted in 200ul water, the concentration would also be 0.5mg/ml.
• To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed).
• Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature.
• It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min.
• Then incubate and develop the two blots in parallel.