84-1083-93　Annexin A2 (ANXA2, annexin A2, ANX2, LIP2, LPC2, CAL1H, LPC2D, ANX2L4, P36, PAP-IV, annexin II, lipocortin II, Annexin II (lipocortin I), annexin II (lipocortin II), calpactin I, heavy polypeptide (p36), annexin II (lipocortin II calpactin I, heavy polypeptide), calpactin I heavy polypeptide, chromobindin 8, lipocortin II, placental anticoagulant protein IV) 100ug　A2295-15C

特徴

• Applications:Suitable for use in ELISA and Western Blot.
• Other applications not tested.
• Recommended Dilution:Peptide ELISA: antibody detection limit dilution 1:4,000.
• Western Blot: 0.1-0.3ug/ml, an ~35kD band observed in lysates of Human cell lines A549 and HeLa (calculated MW of 40.4kD according to NP_001002858.1 and 38.6kD according to NP_004030.1 and NP_001002857.1)
• Staining (0.1ug/ml) of HeLa lysate (RIPA buffer, 35ug total protein per lane).
• Primary incubated for 1 hour.
• Detected by western blot using chemiluminescence
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Peptide Blocking:Corresponding peptide is available for Peptide Blocking studies.
• See A2295-15C-P.
• Antibody is typically 0.5mg/ml and peptide is supplied as a 100ul pellet.
• When peptide is reconstituted in 200ul water, the concentration would also be 0.5mg/ml.
• To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed).
• Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature.
• It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min.
• Then incubate and develop the two blots in parallel.