Membrane-bound form of TNF-a is cleaved by a convertase to generate a secreted 17kD mature protein.
TNF-a converting enzyme, TACE, is a membrane bound metalloprotease-disintegrin.
It also processes other cell surface proteins, including TNF receptor, TGFa, and alpha cleavage of amyloid protein precursor.
Applications:Suitable for use in Immunohistochemistry, ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:Peptide ELISA: 1:128,000Western Blot: 0.1-0.3ug/ml, ~100kD band observed in Human Testis lysates and ~125kD in lysates of cell lines Daudi, HeLa and Jurkat (calculated MW of ~93kD according to NP_003174.3).
Primary incubated for 1 hour.
Preliminary testing was unsuccessful on Mouse and RatImmunohistochemistry: 3.75ug/ml on paraffin embedded Human Skin and ThyroidOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.
Specificity：Recognizes human ADAM 17. Expected to recognize isoform 1 (represented by NP_003174.3) and not isoform 2 (represented by NP_068604).
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to LQRQNRVDSKETEC from the C-terminus of human ADAM17. Species sequence homology: mouse and rat
Shelf Life：1year
Swiss Prot Number：P78536
EU Commodity Code：30021010
商品名：ADAM 17, CT (ADAM17, A Disintegrin and Metalloproteinase Domain 17 (Tumor Necrosis Factor, alpha, Converting Enzyme), CSVP, TACE, cSVP, CD156b, CD156b, MGC71942, TACE, cSVPADAM Metallopeptidase Domain 17, TNF-alpha Converting Enzyme, A Disintegrin and Metalloproteinase Domain 17, A Disintegrin and Metalloproteinase Domain 17 (Tumor Necrosis Factor, alpha, Converting Enzyme), Snake Venom-like Protease) 100ug
