Application(s): Suitable for use in ELISA, Western Blot.
Other applications not tested
Western blot: Approx 60kD and 28kD bands seen in HeLa cell lysates.
Recommended for use at 0.5-2ug/ml.
Overnight incubation is recommended with this antibody.
Please note that the appox 28kD band we observe maybe explained by the isoform described in Bailey et al [J.
Clin.
Endocr.
Metab.
87: 1717-1728, 2002].
Staining (2ug/ml) of HeLa lysate (RIPA buffer, 35ug total protein per lane).
Primary incubated for 12 hour.
Detected by western blot using chemiluminescence
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
Peptide Blocking:Corresponding peptide is available for Peptide Blocking studies.
See A0855-42K-P.
Antibody is typically 0.5mg/ml and peptide is supplied as a 100ul pellet.
When peptide is reconstituted in 200ul water, the concentration would also be 0.5mg/ml.
To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed).
Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature.
It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min.
Then incubate and develop the two blots in parallel.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.
