84-1083-82　Transferrin (Tf) (HRP) 100ul　T8199-04R

特徴

• Transferrin (Tf, human chromosome 3, 679aa), a serum glycoprotein of ~80kD and synthesized in the liver, is the primary protein of inter-organ transport of nonheme iron.
• Tf can bind two iron atoms.
• Tf binds to membrane Transferrin receptors (TfRs) and taken up by endocytosis.
• Iron is released from Tf, within acidic endosomes, into the cytoplasm apparently through the action of DMT1.
• The apoTf-TfR complex is returned to the cell surface, where, apo-Tf dissociates from TfR at the extracellular pH.
• The classical TfR, now termed TfR1, is a homodimeric (95kD subunits) type II membrane glycoprotein that binds two molecules of Tf.
• Human TfR1 (human 760aa; mouse 763aa) has a cytoplasmic domain 1-67aa, 68-88aa TM, and 89-760aa as extracellular domains.
• A monomeric serum form or soluble TfR1 (~80kD) also exists that lacks residues 1-100 aa.
• Recently, a second Tf receptor, TfR2, has been cloned and characterized.
• TfR2 shares 45% identity with TfR1, and 27% with PMSA.
• Several variants of Tf have been identified with varying iron binding ability.
• Applications:Suitable for use in ELISA.
• Western Blot, though not tested, may potentially be used as an application.
• Other applications not tested.
• Recommended Dilutions:ELISA: 1:1000-1:10,000Western Blot:1:1000-1:5000 using ECL.
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Labeled with horseradish peroxidase (HRP).
• Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
• HRP conjugates are sensitive to light.