84-1083-77　Topoisomerase II (DNA) beta 100ul　T8065-23

特徴

• The topology of the DNA is a crucial issue in different biological processes like recombination, replication, transcription, and chromatin remolding.
• DNA topoisomerases are enzymes that control the amount of supercoiling in DNA.
• Without topoisomeras, DNA cannot replicate normally.
• The protein family of DNA topoisomerases is divided into two types (type I and II) based on their mechanism and physical properties.
• While the type I topoisomerases are monomeric and do not require ATP, the type II contain multi-subunits and depend on ATP for their activity.
• The type I enzymes include eukaryotic and bacterial topoisomerase I and III.
• The type II enzymes include bacterial DNA gyrase and topoisomerase IV and the eukaryotic topoisomerase II.
• Type I enzymes introduce transient single strand breaks in DNA, while type II enzymes produce transient double strand breaks in the DNA.
• Topo II plays important roles in synthesis and transcription of DNA as well as chromosomal segregation during mitosis.
• It is reported to be a sensitive and specific marker of late S-, G2- & M-phases in transformed and developmentally regulated normal cells Top II is also implicated in drug resistance of tumor cells.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 2-10ug/mlImmunohistochemistry, Immunofluorescence: 5-10ug/ml; Formalin fixed and paraffin embedded sections (Boiled in 10mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at 4°C for 20 min)
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage, store at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.