84-1083-76　Tie2 (Tunica Internal Endothelial Cell Kinase, Angiopoietin1 Receptor, p140 Tek, Tyrosine Protein Kinase Receptor) 100ug　T5498-71J

特徴

• Embryonic vascular system undergoes a series of complex, highly regulated series of events involving differentiation, migration and association of primitive endothelial cells.
• This process is termed vasculogenesis.
• A further remodeling of the primitive vascular system forms the mature cardiovascular system.
• This process is known as angiogenesis (sprouting of new capillary vessels from pre-existing vasculature).
• A family of receptor tyrosine kinases TIE1 and TIE 2 or Tek has been identified in vascular endothelium and hematopoietic cells.
• Mice lacking TIE 1 or TIE 2 are lethal.
• Ties may represent the earliest endothelial cell lineage marker and may regulate the endothelial cell proliferation, differentiation, and proper patterning during vasculogenesis.
• TIEs appear to be acting downstream of the VEGFRs.
• Tie-2 (human 1124 AA; mouse 1122 aa) is a type 1 membrane receptor protein specifically expressed in developing vascular endothelial cells and their progenitors, angioblasts.
• It is also found in placenta and lung, with lower levels in umbilical vein endothelial cells, brain and kidney.
• Tie-2 extracellular portion (25-279 aa) contains 3 fibronectin type III-like and 2-Ig-like C2-type, and 3-EGF-like domains.
• Applications:Suitable for use in ELISA.
• Western Blot, though not tested, may potentially be used as an application.
• Other applications not tested.
• Recommended Dilutions:ELISA: 1:10,000-1:100,000Western Blot: 1-10ug/ml (ECL)Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage, store at -20°C.
• Aliquots are stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.