84-1083-73　TIE1 (TIE-1, Tyrosine Protein Kinase Receptor, Tunica Internal Endothelial Cell Kinase) 100ul　T5498-65C

特徴

• Embryonic vascular system undergoes a series of complex, highly regulated series of events involving differentiation, migration and association of primitive endothelial cells.
• This process is termed vasculogenesis.
• A further remodeling of the primitive vascular system forms the mature cardiovascular system.
• This process is known as angiogenesis (sprouting of new capillary vessels from pre-existing vasculature).
• A family of receptor tyrosine kinases TIE1 and TIE 2 or Tek has been identified in vascular endothelium and hematopoietic cells.
• Mice lacking TIE 1 or TIE 2 are lethal.
• Ties may represent the earliest endothelial cell lineage marker and may regulate the endothelial cell proliferation, differentiation, and proper patterning during vasculogenesis.
• TIEs appear to be acting downstream of the VEGFRs.
• Tie-1 (human 1138 AA; mouse 1134 aa) is a type 1 membrane receptor protein specifically expressed in developing vascular endothelial cells.
• Tie-1 extracellular portion (25-279 aa) contains 3 fibronectin type III-like and 2 Ig-like C2-type, and 3-EGF-like domains.
• Applications:Suitable for use in Western Blot and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1-10ug/ml using Chemiluminescence technique.
• NIH/3T3 cells can be used as positive control.
• TIE-1 ~135-140kD.
• Recombinant Tie-1 fusion protein migrate as 130-140kD due to glycosylation
• ELISA (1:10K-1:100K; using 50-100ng of control peptide/well)
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with sterile ddH2O or PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.