Superoxide dismutase (SOD) is an enzyme, which thought to play a role in the protection of aerobic cells against oxygen toxicity by catalyzing disputation of superoxide anion (O2-) to H2O2 and O2.
SODs are found in 3 forms and produced by separate genes.
The first isoforms (SOD1, also known as SOD-A, soluble SOD, Cytosolic SOD, Cu-Zn SOD and indophenoloxidase A or IPOA).
Human SOD1 is 154 aa (chromosome 21q22).
It is a homodimer and each subunit can bind 1 copper ion and 1 zinc ion.
SOD1 is cytoplasmic protein.
Defects in SOD1 are the cause of familial amyotrophic lateral sclerosis (FALS) or amyotrophic lateral sclerosis 1 (ALS1 or ALS).
ALS is a degenerative disorder of motorneurons in the cortex, brainstem and spinal cord.
ALS is characterized by muscular weakness and atrophy beginning in the hands and spreading to the forearms and legs.
Death usually occurs within 2 to 5 years.
The familial form of ALS accounts for about 10% of the cases and is transmitted in an autosomal dominant manner.
SOD2 (mitochondrial indophenoloxidaeB, IPO-B, Mn-SOD) is a Mn-containing enzyme found primarily in mitochondria and therefore is not present in erythrocytes.
SOD2 (human 222-aa; chromosome 6q25.3) is a homotetramer.
It binds 1 Mn per subunit.
SOD3 (extracellular-SOD or EC-SOD) is found in extracellular space (blood, lymph, synovial fluids and cerebrospinal fluid).
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilution:ELISA: 0.1-1ug/ml; Control antigen can be used to coat ELISA plates at 1ug/mlWestern Blot: 1-5ug/ml using ECL technique
Optimal dilutions to be determined by the researcher.
Control Peptide:S8060-10H Superoxide Dismutase, Human, Control Peptide
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.1% BSA, before the addition of glycerol to 40%.
Specificity：Recognizes human EC-SOD/SOD3.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Hu
Immunogen：17aa peptide near the C-terminus of human EC-SOD/SOD3 (KLH).
