84-1083-57　Secretin 100ul　S0625-02

特徴

• Glucagon is a member of a multigene family comprising of Secretin, Vasoactive Intestinal Peptide (VIP), Gastric Inhibitory Peptide (GIP) and others like Glicentin and Oxyntomodulin (OXM), which differs from glucagon by C-terminal octapeptide.
• The glucagon precursor contains at least 3 intervening sequences that divide the protein-coding portion into 4 regions corresponding to the signal peptide and part of the N-terminal peptide, the remainder of the N-terminal peptide and glucagon, glucagon-like peptide-1 (GLP1), and GLP2.
• SECR, Secretin is a 27-amino acid hormone (chr 11p15) produced by specific endocrine cells, S cells, located in the mucosa of the proximal small intestine.
• It has been known to be a potent stimulus for the secretion of bicarbonate-rich pancreatic juice.
• Secretion of secretin is stimulated by the presence of either acidic pH or fatty acids in the duodenum.
• Secretin stimulates ductal bile secretion by directly interacting with cholangiocytes, promotes osmotic water movement in cholangiocytes by inducing the exocytic insertion of AQP1 into plasma membranes.
• Applications:Suitable for use in ELISA.
• Western Blot, though not tested, may potentially be used as an application.
• Other applications not tested.
• Recommended Dilution:ELISA: 0.5-1ug/ml; Control peptide can be used to coat ELISA plates at 1ug/ml.
• See S0625-02A
• Western Blot: 1-10ug/ml using ECL technique
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with sterile ddH2O or PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.