Sclerostin (213aa in rat and human (chr 17q12) and 211aa in mouse) is a novel secreted osteoclast-derived BMP antagonist with unique ligand specificity.
It negatively regulates the formation of bone by repressing the differentiation and/or function of osteoblasts induced by BMPs.
Since sclerostin expression is confined to the bone resorbing osteoclast, it provides a mechanism whereby bone apposition is inhibited in the vicinity of resorption; indicating the role of sclerostin in bone remodeling and links bone resorption and bone apposition.
Defects in SOST are the cause of sclerosteosis, a progressive sclerosing bone dysplasia.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilution:ELISA: 0.5-1ug/mlWestern Blot: 1-10ug/ml using ECL technique
Optimal dilutions to be determined by the researcher.
Recommended Control Peptide:S0501-05A: Sclerostin, Human, Control Peptide.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.1% BSA.
Specificity：Recognizes human Sclerostin.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide, 17aa ~C-terminus corresponding to human Sclerostin. Species Sequence Homology: rat - 94%, mouse - 88%.
