84-1083-53　PTX3 (Long Pentraxin, TSG14) 100ul　P9183-04B

特徴

• Pentraxins are a family of proteins emerging from genes that are conserved in their carboxy-terminal halves a pentraxin domain and are prototypical acute phase proteins with acquired novel amino-terminal domains.
• Pentraxins, include C reactive protein (CRP) and Serum Amyloid P component (SAP), which serve as indicators of inflammatory reactions as a result of the exposure of liver cells to cytokines, mainly interleukin-6 (IL-6).
• PTX3 or TSG14 or long pentraxin (human mature chain 364 aa, chromosome 3q25; ~43 kda ) is structurally related but different form classic pentraxins.
• PTX3 was clones as an IL-1 inducible gene in endothelial cells, and as TNF-inducible gene in fibroblasts called TSG14.
• The C-terminal half of PTX3 aligns with CRP and SAP, but the N-terminal half is unique.
• PTX3 is induced by LPS, IL-1 and TNF-alpha, but not IL-6.
• During an acute phase response induced by LPS, PTX3 is expressed in many organs (heart, skeletal muscle).
• PTX3 expression may be indicative of its role in cardiovascular and inflammatory pathology.
• Applications:Suitable for use in ELISA.
• Western Blot, though not tested, may potentially be used as an application.
• Other applications not tested.
• Recommended Dilution:ELISA: 0.5-1ug/ml; Control peptide can be used to coat ELISA plates at 1ug/ml.
• See P9183-04C
• Western Blot: 1-10ug/ml using ECL technique
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with sterile ddH2O or PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.