Oxygen is absolutely critical for the survival of mammalian cells.
Hypoxia induced factor (HIF) is a transcriptional complex that plays a central role in mammalian oxygen homeostasis.
There are 3-types of alpha subunits (HIF1-3alpha) and one HIF-beta subunits.
However, the three HIF-alpha subunits are regulated by oxygen in a similar fashion, i.e.
by regulated stabilization of the alpha-subunits.
Under normal conditions, HIF-alpha Prolines are hydroxylated at Pro-402 and Pro-564.
This allows binding of von Hippel-Lindau (VHL), the substrate recognition component of the E3 ubiquinated ligase complex, subsequent ubiquitination and degradation of HIF-alpha by the proteasome.
Under hypoxic conditions, hydroxylation of HIF-alpha is inhibited and this prevents HIF-alpha degradation.
The enzymes responsible for HIF-hydroxylation are known as HIF-prolyl hydroxylases (PHD1-3 or HPH1, HPH2, and HPH3).
The three PHDs have been identified to hydroxylate the motif, LXXLAP* with *P being the hydroxyproline.
PHD3 (human 333-aa, chromosome 14; also known as EGL9, C.
ELEGANS, HOMOLOG OF, 3, EGLN3, PROLYL HYDROXYLASE DOMAIN-CONTAINING PROTEIN 3;PHD3, HIF-PROLYL HYDROXYLASE 3; HIFPH3) Widely expressed at low levels.
Expressed at higher levels in heart (cardiac myocytes, aortic endothelial cells and coronary artery smooth muscle) and placenta.
It is activated under hypoxia.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilution:ELISA: 1:10,000-1:100,000; Using 50-100ng of control peptide/well.
See P3375-07A
Western Blot: 1-5ug/ml using ECL technique
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile ddH2O or PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
Specificity：Recognizes human PHD3. Species Sequence Homology: Mouse, rat - 87%.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Hu
Immunogen：16aa peptide from the C-terminal region of human PHD3/EGLN3.
