84-1083-49　PHD1-3, pan (HIF-prolyl Hydroxylase 1-3) 100ug　P3373A

特徴

• Oxygen is absolutely critical for the survival of mammalian cells.
• Hypoxia induced factor (HIF) is a transcriptional complex that plays a central role in mammalian oxygen homeostasis.
• There are 3-types of alpha subunits (HIF1-3alpha) and one HIF-beta subunits.
• However, the three HIF-alpha subunits are regulated by oxygen in a similar fashion, i.e.
• by regulated stabilization of the alpha-subunits.
• Under normal conditions, HIF-alpha Prolines are hydroxylated at Pro-402 and Pro-564.
• This allows binding of von Hippel-Lindau (VHL), the substrate recognition component of the E3 ubiquinated ligase complex, subsequent ubiquitination and degradation of HIF-alpha by the proteasome.
• Under hypoxic conditions, hydroxylation of HIF-alpha is inhibited and this prevents HIF-alpha degradation.
• The enzymes responsible for HIF-hydroxylation are known as HIF-prolyl hydroxylases (PHD1-3 or HPH1, HPH2, and HPH3).
• The three PHDs have been identified to hydroxylate the motif, LXXLAP* with *P being the hydroxyproline.
• Applications:Suitable for use in ELISA.
• Western Blot, though not tested, may potentially be used as an application.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:10,000-1:100,000; Using 50-100ng of P3373B control peptide/well.
• Western Blot: 1-5ug/ml.
• Using ECL technique
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for at least 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.