Pentraxins are a family of proteins emerging from genes that are conserved in their carboxy-terminal halves a pentraxin domain and are prototypical acute phase proteins with acquired novel amino-terminal domains.
Pentraxins, include C reactive protein (CRP) and Serum Amyloid P component (SAP), which serve as indicators of inflammatory reactions as a result of the exposure of liver cells to cytokines, mainly interleukin-6 (IL-6).
Neuronal Pentraxins: A new family of putative integral membrane pentraxins or Neuronal pentraxins identified through interaction with a presynaptic snake venom toxin taipoxin.
NPX1, NPX2 and NPXR (Neuronal Pentraxin Receptor), the three neuronal pentraxins represent a novel neuronal uptake pathway that may function during synapse formation and remodeling.
The N-terminal half of neuronal pentraxins are 20-30% identical to previously identified pentraxins (CRP and SAP), the three neuronal pentraxins are 50% identical to each other and are significantly larger than the classical pentraxins (>50 versus 30kDa), suggesting that they may have additional novel functions.
NPXR1 (Neuronal Pentraxin Receptor 1) a 493aa protein in mouse, 494 in rat, and 499aa in human.
The predominant form of NPXR contains a putative NH2 terminal transmembrane and all forms are glycosylated.
It shows 49 and 48% homology with NPX1 and NPX2 respectively.
NPXR message is expressed in neuronal regions that express NPX1 and NPX2.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilution:ELISA: 0.5-1ug/ml; Control peptide can be used to coat ELISA plates at 1ug/ml.
See N5379-14A
Western Blot: 1-10ug/ml using ECL technique
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
Specificity：Recognizes mouse NPXR1.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Mo
Immunogen：14aa peptide from mouse NPXR1. Location: ~C-terminus. Species Sequence Homology: Human, rat - 100%.
