84-1083-29　Noggin 100ul　N3100-05

特徴

• Cell-cell communication is mediated by secreted proteins such as Notch, Hedgehog, Wingless, Fibroblast Growth Factor (FGF), Epidermal Growth Factor (EFG) and Transforming Growth Factor-beta (TGF-beta) and their respective cell surface receptors.
• The TGF-beta superfamily includes TGF-beta, Activin, Nodal, Mullerian inhibiting substance (MIS), growth and differentiation factor (GDF) and bone morphogenetic proteins (BMPs).
• BMPs have been implicated in limb growth and patterning.
• The joints are formed after initial cartilage condensation followed by cell death and cavitation.
• The BMPs function by binding to a receptor complex that is found on all normal cells and is composed of type-I and –II receptors.
• BMP activities are modulated through gene expression, protein processing and by interaction with antagonists.
• The interplay between BMPs and their antagonists governs developmental and cellular processes as diverse as establishment of the embryonic dorsal-ventral axis, induction of neuronal tissue, formation of joints in the skeletal system and the neurogenesis in the adult brain.
• The BMP anagonists include the Differential screening-selected gene Aberrant in Neuroblastoma (DAN) family members (DAN, Cerberus, Gremlin), Chordin, Noggin and SOG.
• Noggin inhibits BMP signaling by blocking the molecular interfaces of the binding epitopes for both type-I and –II receptors.
• Noggin is a homodimer (~32kDa) of two monomeric units linked together by a disulfide bond.
• The monomeric precursor (232aa, human and mouse) is encoded by NOG gene, mapped at human chromosome 17.
• The mature protein (28-232aa) is secreted as a glycosylated dimer which binds to BMPs including BMP-2, -4 and–7.
• The structure of Noggin is very similar to BMP-7.
• Mutation in Noggin gene leads to skeletal dysplasias characterized by joint fusions.
• Applications:Suitable for use in Western Blot and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1-10ug/ml using ECL technique.
• See N3100-05B for positive control
• ELISA: 0.5-1ug/ml; Control peptide can be used to coat ELISA plates at 1ug/ml.
• See N3100-05A
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage, store at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.