84-1083-25　Neuropeptide Y5 Receptor (NPY 5R) 100ul　N2176-66

特徴

• Neuropeptide Y (NPY), a neuropeptide colocalized and abundantly released with the neurotransmitter norepinephrine in nervous system, synthesized by postnatal olfactory epithelium of sustentacular cells.
• It promotes the proliferation of postnatal neuronal precursor cells and exhibits a diverse range of important physiologic activities, including effects on psychomotor activity, food intake, regulation of central endocrine secretion, and potent vasoactive effects on the cardiovascular system.
• NPY a 97aa peptide in mouse, human (chr 7p15.1) and 98aa in rat has a mol.
• wt of 11kD.
• NPY shows sequence homology to Peptide YY (PYY) and over 50% homology in amino acid and nucleotide sequence to Pancreatic Polypeptide (PNP or PPY), all of these homologous peptides has a 36aa mature peptide characterized by a hairpin loop.
• NPY5R, a 466aa in mouse, 456aa in rat and 455 in human (chr 4q31-q32), it is the receptor for NPY and PYY.
• The activity of this receptor is mediated by G proteins that inhibit Adenylate cyclase activity.
• Expressed in brain and hypothalamus.
• Applications:Suitable for use in ELISA.
• Western Blot, though not tested, may potentially be used as an application.
• Other applications not tested.
• Recommended Dilution:ELISA: 0.5-1ug/ml; Control peptide can be used to coat ELISA plates at 1ug/ml.
• See N2176-66B
• Western Blot: 1-10ug/ml using ECL technique
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with sterile ddH2O or PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.