Neuropeptide Y (NPY), a neuropeptide colocalized and abundantly released with the neurotransmitter norepinephrine in nervous system, synthesized by postnatal olfactory epithelium of sustentacular cells.
It promotes the proliferation of postnatal neuronal precursor cells and exhibits a diverse range of important physiologic activities, including effects on psychomotor activity, food intake, regulation of central endocrine secretion, and potent vasoactive effects on the cardiovascular system.
NPY a 97aa peptide in mouse, human (chr 7p15.1) and 98aa in rat has a mol.
wt of 11kD.
NPY shows sequence homology to Peptide YY (PYY) and over 50% homology in amino acid and nucleotide sequence to Pancreatic Polypeptide (PNP or PPY), all of these homologous peptides has a 36aa mature peptide characterized by a hairpin loop.
NPY1R, 382aa in mouse, rat and 384aa in human (chr 4q31.3), is mainly expressed in brain, heart, kidney and spleen.
It is the receptor for Neuropeptide Y and Peptide YY.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilution:ELISA: 0.5-1ug/ml; Control peptide can be used to coat ELISA plates at 1ug/ml.
See N2176-50E
Western Blot: 1-10ug/ml using ECL technique
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.1% BSA.
Specificity：Recognizes mouse NPY 1R.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Mo
Immunogen：A synthetic peptide corresponding to a 15aa sequence from mouse NPY 1R (KLH). Location: ~N-terminus, Extracellular domain. Species Sequence Homology: Human - 86%; rat - 80%.
