Many tissues such as muscle, skin, liver, and peripheral nerve, have remarkable ability to repair and regrow after injury.
However, the CNS (brain and spinal cord) is limited in its ability to repair or regrowth causing permanent brain damage or paralyses.
Most recently an inhibitory myelin protein, Nogo (Neurite outgrowth), has been cloned and characterized.
It may help block the regeneration of the CNS.
Recently, a brain specific leucine-rich-repeat protein with high affinity for soluble Nogo-66, termed Nogo receptor (Ngr) has been cloned and characterized.
Cleavage of Ngr from the axonal surface renders neurons insensitive to Nogo-66.
Ngr expression is sufficient to impart Nogo-66 axonal inhibition to unresponsive neurons.
Ngr is expressed in brain and lower levels are also detected in hear and kidney but not in other peripheral tissues.
Additional proteins homologous to Ngr have now been identified and designated NGR2 or NgRH1 (nogo-66 receptor homolog-1; human and rat 420 aa; chromosome 11q12.1 ) and Ngr3 or NgRH2 (nogo-66 receptor homolog-2; rat 438 aa; mouse, 445 aa; human 441 aa, chromosome 17p13.3).
There is approx.
45% sequence homology between the NGR1-3.
Despite high degree of sequence homology and similar protein topology, the NGR2 and NGR3 do not bind Nogo, MAG, Omgp or NGR1.
The identity of other natural ligands for NGR2-3, and the physiological roles of NGR2-3 are unknown.
Both NGR2 and NGR3 are expressed in the brain.
The NGR2-3 proteins appeared as ~45 and ~65 kda protein (glycosylated).
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilution:ELISA: 1:10,000-1:100,000; Using 50-100ng of control peptide/well.
See N2116-98B
Western Blot: 1:1000-1:5000; Using ECL
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
Specificity：Recognizes human Ngr3.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Hu
Immunogen：18aa peptide of human Ngr3. Location: ~C-terminal. Species Sequence Homology: Mouse, rat - 100%.
