84-1083-06　NCKX1 (K+-dependent, Na Ca Exchanger 1) 100ul　N0559-02D

特徴

• Ca2+ plays a critical role in intracellular signaling.
• Intracellular Ca2+ levels are tightly controlled by continuous removal of Ca2+ via ATP-driven Ca2+ pump in the endoplasmic reticulum and plasma membrane, and the Na+/Ca2+ exchangers (NCX), in the plasma membrane.
• NCX can move Ca2+ either into or out of cells, depending on the net Na+, Ca2+, and K+ gradient across the membrane.
• In most cells, 3 Na+ are exchanged for 1 Ca2+.
• Several genes code for the 3 NCX (NCX1, NCX2, and NCX3), and three in the NCKX family (NCKX1, NCKX2, NCKX3).
• NCX share significant sequence homology (~70%), display 11 TM domains, a large central, intracellular hydrophilic regulatory loop between TM5 and 6, extracellular N-terminus and cytoplasmic C-terminus.
• The N-terminal signal peptide is cleaved off from the mature exchanger protein.
• In vertebrate photoreceptors, some neurons, and certain other cells, 3 K+ are transported in the same direction as Ca2+, with coupling ratio of 4 Na+ to 1 Ca2+ plus 1 K+ by new class of exchangers called NCKX for K+-dependent Na/Ca+2 exchangers.
• NCKX share the general topology and functional properties of NCX proteins.
• Rod exchangers also contains a large hydrophilic segment at the its N-terminus that is not found in NCX.
• NCKX1 (human 1099/1081 aa, bovine 1199 aa) is expressed in retinal rod cells.
• NCKX2 (rat 670 aa) shares 80% identity with NCKX1.
• The two hydrophilic loops are also much shorter in NKKX2 than in NCKX1, accounting largely for the difference between the size of the two proteins.
• NCKX2 is expressed in various regions of the brain (striatum, parietal cortex, cerebellum, hippocampus, and thalamus).
• A third member of NCKX family, namely NCKX3 (rat 624 aa, mouse 595, and human 644/625) has been cloned and characterized.
• Human NCKX3 is most abundant in the brain (thalamic nuclei, hippocampal CA1 neurons, layer IV of the cerebral cortex).
• It is also expressed at lower levels in aorta, uterus, and intestine.
• Applications:Suitable for use in ELISA.
• Western Blot, though not tested, may potentially be used as an application.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:10,000-1:50,000; Control peptide can be used to coat ELISA plates at 1ug/ml.
• See N0559-02E
• Western Blot: 1:1000-1:5000 using ECL technique
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage, store at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.