Bicarbonate, along with CO2, is the major pH buffer of biological fluids.
A great majority of HCO3-reabsorption occurs via trans-cellular coupling of the luminal Na-H+-exchanger 3 and Na+-H+-ATPase with the basolateral Na+-HCO3- cotransporters (NBC).
Several related proteins constitute the emerging NBC family (NBC1-3) of membrane cotransporters that are found in a variety of epithelial and non-epithelial tissues, and may be tissue specific.
Physiologically, NBC is electrogenic, Na+ and HCO3- dependent, Cl- independent, and inhibited by stilbenes (DIDS and SITS).
The NBC family of proteins are 30-35% related to anion exchangers (AE2 and AE3; SLC4A1-SLC4A3) and display the same protein topology: (a) At least 10 TM domains with both the N and C-termini predicted to be intracellular, (b) presence of a large, glycosylated, extracellular loop between TM5 and TM6; and (c) the lysine residues are conserved at predicted DIDS-reactive sites.
NBC1 (human, rat, mouse 1035 aa, also called NBC-1A/1B, hkNBC, rkNBC, pNBC, hhNBC, SLC4A4) was initially cloned from human Kidney.
NBC1 is 30-35% identical with AEs.
It is strongly expressed in the kidney and pancreas.
The rat kidney NBC1 (rkNBC1; 1035 aa) is 86% identical to hkNBC1.
Pancreatic NBC (pNBC/hhNBC/SLC4A5, human 1079 aa) has a unique n-terminal 85 aa sequence replacing the 41-aa in kNBC.
pNBC is also more widely expressed (pancreas, thyroid, heart, and brain).
Applications:Suitable for use in Western Blot and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1-10ug/ml; using Chemiluminescence technique
ELISA: 1:10,000-1:100,000; Using 50-100ng of control peptide/well.
See N0536-06
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：rat
Grade：Serum
Purity：Serum
Form：Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
Specificity：Recognizes rat NBC1. Antibodies will recognize both the kidney and pancreatic/heart isoforms of NBC1. Species Sequence Homology: Human, mouse, rabbit - 100%; Frog - 75%.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Rt
Immunogen：20aa synthetic peptide within the cytoplasmic, N-terminus of rat NBC1, coupled with KLH. This region is also shared 100% with pNBC/PSLC4A5. No significant sequence homology was found with NBC2 or NBC3.
