84-1083-02　［取扱停止］NaPi-2A (Npt IIa) 100ug　N0035-24L

特徴

• Inorganic phosphate (Pi) levels are hormonally regulated that affects the physiological activity of bone, kidney, and small intestine.
• Majority of the Pi is absorbed in the small intestine and reabsorbed in the proximal tubules in the kidney.
• At least 4 groups of structurally and functionally related proteins are in involved in Pi transport: Type I-related NaPi transporters designated NPT1, Npt1, and NaPi-1 respectively in humans, mouse, and rabbit are expressed in the kidney and liver.
• Its expression and activity are not regulated by Pi deprivation or parathyroid hormone (PTH) and its role in Pi-homeostasis is not clear.
• Type IIa-related cotransporters, designated NaPi-2 in rat, NaPi-3 or NPT2 in humans, NaPi-4 in opposum, NaPi-5 in flounder vessel, NaPi-6 or Npt-2 in mouse, and NaPi-7 in rabbit, is the primary target for Pi regulation by dietary, hormonal, and tubular Pi reabsorption.
• Deletion of Npt2 gene produces severe Pi wasting.
• Applications:Suitable for use in Western Blot and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1000-1:5000; using Chemiluminescence technique.
• NPT2a apparent mol wt is 80-90kD, and under reducing conditions 2 bands of 40-50kD are observed.
• It may also form multimeric structures (170 and 200kD)
• ELISA: Control peptide can be used to coat ELISA plates at 1ug/ml.
• See N0035-24H
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage, store at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.